Fig. 1.

Assessment of peripheral RB51-specific, IFN-γ production by CD4⁺ T cells following VPEAR and RB51 vaccination and RB51 boost. (A) Representative dot plots showing FSC vs. SSC profile, gating strategy for singlet discrimination, viability assessment, and CD4 and intracellular IFN-γ staining on bovine PBMC. Time course showing the frequency of IFN-γ⁺ CD4⁺ T cells in PBMC from naïve (open bars), VPEAR- (black bars) or RB51-vaccinated (gray bars) animals following (B) initial vaccination and (C) post-RB51 boost. (D) Frequency of IFN-γ⁺ CD4⁺ T cells in PBMC following stimulation with pokeweed mitogen as a positive control for IFN-γ production. Lower-case letters denote statistical significance between treatment groups at each individual timepoint, indicated by a p value ≤0.05.