Wnt1-Cre;Prmt1fl/fl mice exhibit multiple craniofacial malformations at birth.
(A, B) Sagittal views of newborn control
and Wnt1-Cre;Prmt1fl/fl heads. Intraoral views of the (C, D)
palate and (E, F) tongue of control and
Wnt1-Cre;Prmt1fl/fl newborn mice. Dashed line in panel D indicates cleft palate.
(G, H) Sagittal views of micro–computed
tomography scans of control and Wnt1-Cre;Prmt1fl/fl newborn heads. Arrow in panel H indicates cleft palate.
Three-dimensional reconstructions from micro–computed tomography scans
of control and Wnt1-Cre;Prmt1fl/fl (I, J) heads and (K,
L) mandibles. (M, N) Alizarin
red/alcian blue staining of control and Wnt1-Cre;Prmt1fl/fl heads. (O) Normalized head length, mandible length,
ratio between mandible length and head length, tongue length, and ratio
between tongue length and head length. Error bars indicate SD.
*P < 0.05. n = 4. Scale bar = 1
mm.