Figure 10.
The effect of ulipristal acetate (UPA) versus UPA/1,25-dihydroxyvitamin D3 (VitD3) combination treatment on vitamin D receptor (VDR) expression in human UF cell line (HuLM). The 5 × 105 HuLM cells were seeded in 10-cm plates, serum starved overnight, and treated with UPA 100 nM in the presence or absence of 100 nM VitD3 for 2 days. Ethanol was used as vehicle control. A, Western blot analysis was performed using anti-VDR antibody. The intensity of each protein signal was quantified and normalized to the corresponding β-actin and presented in the graph as mean ± standard deviation (SD). B, The messenger RNA (mRNA) level of gene coding for VDR was measured by quantitative real-time polymerase chain reaction (qRT-PCR) analysis using gene-specific sense and antisense primers as described in Materials and Methods. The mRNA levels were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and normalized values used to generate the graph. Data are presented as mean ± standard error of the mean (SEM) of triplicate measurement. *P < .05. The experiments were repeated twice.