Table 2.
Human Forward and Reverse Primers Sequences for qRT-PCR.
| Gene | Forward Primer Sequence | Reverse Primer Sequence | |
|---|---|---|---|
| CDK1 | Cyclin-dependent kinase 1 | 5′-GTCAGCTCGTTACTCAACTC-3′ | 5′-GCTAGGCTTCCTGGTTTC-3′ |
| PCNA | Proliferating cell nuclear antigen | 5′-GCAAGTGGAGAACTTGGAAATG-3′ | 5′-GCCTAAGATCCTTCTTCATCCTC-3′ |
| BCL2 | B-cell lymphoma 2 | 5′-TTGTGGCCTTCTTTGAGTTCGGTG-3′ | 5′-GGTGCCGGTTCAGGTACTCAGTCA-3′ |
| PARP | Poly (ADP-ribose) polymerase | 5′-AGGTCAAGGAGGAAGGTATCA-3′ | 5′-CAGAGTGTTCCAGTCCAGAATC-3′ |
| TGF-β3 | Transforming growth factor β 3 | 5′-CACACAGTCCGCTTCTTC-3′ | 5′-AGAAGAGGGTGGAAGCC-3′ |
| FMDN | Fibromodulin | 5′-TTTTATCATCGTTCTGCCTTCATG-3′ | 5′-TGTTTGCGGGACCTTAGGAA-3′ |
| BGN | Biglycan | 5′-TTGCCCCCAAACCTGTACTG-3′ | 5′-AAAACCGGTGTCTGGGACTCT-3′ |
| VSCN | Versican | 5′-TGGAATGATGTTCCCTGCAA-3′ | 5′-AAGGTCTTGGCATTTTCTACAACAG-3′ |
| FN | Fibronectin | 5′-AAACCAATTCTTGGAGCAGG-3′ | 5′-CCATAAAGGGCAACCAAGAG-3′ |
| COL1A | Collagen type I | 5′-GGCGACAGAGGCATAAAG-3′ | 5′-TCATCAGCCCGGTAGTAG-3′ |
| MMP 2 | Matrix metalloproteinase 2 | 5′-TCTACTCAGCCAGCACCCTGGA-3′ | 5′-TGCAGGTCCACGACGGCATCCA-3′ |
| MMP 9 | Matrix metalloproteinase 9 | 5′-TTCGACGTGAAGGCGCAGATGGT-3′ | 5′-TAGGTCACGTAGCCCACTTGGTC-3′ |
| TIMP 1 | Tissue inhibitor of matrix metalloproteinase 1 | 5′-TGGACTCTTGCACATCACTACCTGC-3′ | 5′-AGGCAAGGTGACGGGACTGGAA-3′ |
| NFKB1 | Nuclear factor kappa-B | 5′-CCCTGACCTTGCCTATTTG-3′ | 5′-GCTGTCCTGTCCATTCTTAC-3′ |
| RANKL | Receptor activator of nuclear factor kappa-B ligand | 5′-AGAAAGCGATGGTGGATGG-3′ | 5′-GGGATGTCGGTGGCATTA-3′ |
| IL-1 β | Interleukin 1 beta | 5′-ACAGATGAAGTGCTCCTTCCA-3′ | 5′-GTCGGAGATTCGTAGCTGGAT-3′ |
| IL-6 | Interleukin 6 | 5′-TGCAATAACCACCCCTGACC-3′ | 5′-GTGCCCATGCTACATTTGCC-3′ |
| IL-8 | Interleukin 8 | 5′-GACCACACTGCGCCAACAC-3′ | 5′-CTTCTCCACAACCCTCTGCAC-3′ |
| TNF-α | Tumor necrosis factor α | 5′-CTGGGCAGGTCTACTTTGGG-3′ | 5′-CTGGAGGCCCCAGTTTGAAT-3′ |
| EGF | Epidermal growth factor | 5′-AGCCCTGAAGTGGATAGAG-3′ | 5′-GCACCGGCAGACATAAC-3′ |
| EGFR | Epidermal growth factor receptor | 5′-CGCAAGTGTAAGAAGTGCGAA-3′ | 5′-CGTAGCATTTATGGAGAGTGAGTCT-3′ |
| VEGF-A | Vascular endothelial growth factor | 5′-CTACCTCCACCATGCCAAGT-3′ | 5′-GCAGTAGCTGCGCTGATAGA-3′ |
| ADM | Adrenomedullin | 5′-CGAAAGAAGTGGAATAAGTGGG-3′ | 5′-TCAAGTAATAGTGAGGCTTGCG-3′ |
| PDFG-A | Platelet-derived growth factor A | 5′-CACACCTCCTCGCTGTAGTATTTA-3′ | 5′-GTTATCGGTGTAAATGTCATCCAA-3′ |
| PDFG-B | Platelet-derived growth factor B | 5′-TCCCGAGGAGCTTTATGAGA-3′ | 5′-ACTGCACGTTGCGGTTGT-3′ |
| VDR | Vitamin D receptor | 5′- ATGCCATCTGCATCGTCTC-3′ | 5′-GCACCGCACAGGCTGTCCTA-3′ |
Abbreviation: qRT-PCR, quantitative real-time polymerase chain reaction.