Analysis of osteoblast activity in TMJ condylar subchondral bone. (A)
Osteocalcin immunohistochemical (IHC) staining of TMJ subchondral bone from 12- and
32-week control and experimental groups. (B) The number of
osteocalcin-positive cells within the selected area was counted. (C)
Real-time PCR analysis of expression of ALP, Col1a1, Col1a2, the
ratio of Col1a1/Col1a2, osteocalcin, DMP-1, and VEGF
genes from the subchondral bone of the mandibular condylar head in 12- and 32-week
control and experimental groups (*p < 0.05, **p
< 0.01).