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. 2019 Aug 29;11(9):e10061. doi: 10.15252/emmm.201810061

Figure 2. PF‐PH patients exhibit increased proliferation of vascular wall cells and increased Slug expression.

Figure 2

  • A
    Representative images of Ki67‐positive pulmonary vascular endothelial cells (EC, vWF in green) and smooth muscle cells (SMC, aSMA in white) and quantification (* versus PF, *P < 0.05, **P < 0.01).
  • B, C
    Relative expression of Slug mRNA (B) and protein (C) normalized to GAPDH in PF and PF‐PH patients.
  • D
    Representative images of Slug (red) expression in bronchial epithelial cells (EpC), fibroblasts (Fib) (vimentin in green), macrophages (Mф, CD68 in green), pulmonary vascular endothelial cells (EC, von Willebrand factor in green), and pulmonary vascular smooth muscle cells (SMC, aSMA in white); and quantification.
  • E
    Relative CD68 mRNA expression normalized to GAPDH.
  • F
    Representative images of Slug (red) expression in macrophages (Mф, CD68 in green) in non‐fibrotic and fibrotic areas of the lung in PF and PF‐PH patients and quantification.
Data information: Values are expressed as mean ± SEM. The number of samples per group for each experiment is included within each bar graph. Statistical test: panels (A–E): t‐test; panel (F): ANOVA (* versus PF; *P < 0.05).