Figure 3. Online lung microarray analysis revealed PIP as one of the Slug transcriptional targets mediating vascular cell proliferation in PF‐PH.

• A
  Heatmap of the 20 genes upregulated in GSE24988 microarray and known to be Slug transcriptional target.
• B
  Venn diagram showing the overlap between Slug targets implicated in cell proliferation and known to be extracellular.
• C
  Heatmap of the seven transcriptional targets of Slug known to promote proliferation and to be extracellular.
• D, E
  Relative expression of prolactin‐induced protein (PIP) mRNA (D) and protein (E) expression normalized to GAPDH in PF and PF‐PH patients.
• F
  Representative images of PIP immunohistochemistry showing its extracellular localization and quantification.
• G–J
  Proliferation assay on healthy fibroblast (G), pulmonary arterial EC (H), and healthy pulmonary arterial SMC (I and J) in the presence or absence of PIP.

Data information: Values are expressed as mean ± SEM. The number of samples per group for each experiment is included within each bar graph. Statistical test: panels (D–F): t‐test; panels (G–J): ANOVA (*P < 0.05, **P < 0.01 ***P < 0.001).