Figure EV3. Median Z‐projections of IHC and OHC stereocilia tips from the second tallest row.

A pseudo‐stack comprising of ≥ 80 images of individual stereocilia tips extracted from scanning electron micrographs prepared from P8 wild‐type and clarinet mutant mice. The Adobe Photoshop “Auto tone” function was used to increase the contrast of each median projection, followed by pseudo‐colouring to highlight the edges of the projected “averaged” stereocilia tips. These image adjustments allow an accurate outline of each median projection to be drawn. For both inner and outer hair cells, the tips of the stereocilia from the second tallest row of Clrn2 ^{clarinet/clarinet} mice appear more rounded than those of their wild‐type (Clrn2 ^+/+) littermates.