Figure 3.

Analysis of hepatic n-3 and n-6 PUFAs. Quantitative analysis of hepatic DHA (a), EPA (b), LA (c), and AA (d) content of STZ/HFD-treated mice fed HFDs differing in n-3/n-6 PUFA ratios (1:8, 1:1, and 5:1) given as percentage of total fatty acids (n = 10 per group). Values are given as mean ± SEM. Differences between the groups were assessed by two-way ANOVA followed by Tukey’s range test within each time point. *p < 0.05 versus 1:8 fed mice, **p < 0.01 versus 1:8 fed mice, ****p < 0.0001 versus 1:8 fed mice, ^####p < 0.0001 versus 1:1 fed mice.

AA, arachidonic acid; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid; HFD, high-fat diet; LA, linoleic acid; SEM, standard error of the mean; STZ, streptozotocin.