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. 2019 Mar 6;28(8):985–1001. doi: 10.1177/0963689719834873

Fig. 4.

Fig. 4.

Different effects of chemical inhibitor, genetic-knockdown and - overexpression on the protein levels of NHE1 in brain tissues of rats at 24 h after SAH. (A) Western blot analysis show that the protein levels of NHE1 were significantly decreased in HOE642 and Si-NHE1 treatment groups, whereas levels increased in the SAH + Over-NHE1 group. (B) Quantification of relative protein levels of NHE1 in various groups are shown. (C) The interactions of NHE1 and CHP1 in brain tissues of rats at each group were also tested by IP analysis. Input, 5% of extract before IP. (D) Quantification of protein levels of NHE1 interacted with CHP1 at various groups. All data are presented as mean ± SEM; *p < 0.05 compared with Sham group; ns, no significant difference vs. SAH group; # p < 0.05 vs. SAH + Vehicle group; & p < 0.05 vs. SAH + Si-NC group; @ p < 0.05 vs. SAH + Vector group; n = 6.