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. 2019 Aug 15;29:1–11. doi: 10.1016/j.molmet.2019.08.009

Figure 2.

Figure 2

Effects of RYGB on TRPA1 expression and currents in β-cells. (A, B) TRPA1 mRNA (A) and protein (B) in islets isolated from Wistar, GK-Sham, and GK-RYGB rats. A representative immunoblot is shown on the top (B). β-actin was used as internal and loading control. Intensities were quantified and normalized against the level of actin and expressed as the percentage of protein abundance of Wistar islets. Data are means ± S.E.M. of 5 experiments. *, p < 0.05; **, p < 0.01. (C–E) Representative recording of AITC (100 μM)-induced currents in β-cells isolated from Wistar (C), GK-Sham (D), and GK-RYGB (E) rats. (F) Mean (±S.E.M.) of AITC-sensitive currents of β-cells from Wistar (white bar, n = 10), GK -Sham (grey bar, n = 10), and GK-RYGB (dark bar, n = 13) rats. **, p < 0.01. Statistics in A, B and F: One-way ANOVA with LSD post hoc test.