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. 2019 Aug 15;29:1–11. doi: 10.1016/j.molmet.2019.08.009

Figure 5.

Figure 5

Lack of RYGB-induced TRPA1 expression in islets from FXR−/- mice. (A) Plasma total bile acid in GK-Sham (white bar) and GK-RYGB (grey bar) rats. Data are means ± S.E.M. n = 3. Two-sided unpaired t-test was used for statistical analysis. *, p < 0.05. (B) Plasma CDCA in GK-Sham (white bar) and GK-RYGB (grey bar) rats. Data are means ± S.E.M. n = 3. Data were analyzed by two-sided unpaired t-test. *, p < 0.05. (C) A representative immunoblot of TRPA1 protein expression in islets from FXR+/+ and FXR−/- mice after RYGB or sham surgery. β-actin was used as internal and loading control. (D) TRPA1 protein was quantified and normalized against the level of β-actin and expressed as the percentage of protein abundance of FXR+/+ islets. Data are means ± S.E.M. of 4 independent experiments. Data were analyzed by One-way ANOVA (LSD). *, p < 0.05.