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. 2019 Aug 30;10:2073. doi: 10.3389/fimmu.2019.02073

Figure 3.

Figure 3

Functional assessment of NK-92MI cells after their association with MNPs and in vitro magnetic retention. (A) Conjugation kinetics of NK-92MI cells, after being treated with MNPs, with the K562 cell line, co-incubated in a 1:2 ratio. (B) Cytolytic activity of NK-92MI cells, after treatment with different concentrations of MNPs, against the K562 cell line, co-incubated in different ratios for 4 h. (C) Migratory capacity of NK-92MI cells after treatment with MNPs in response to a specific chemotactic gradient and in the presence or absence of an EMF in the same direction. The results were normalized against a control well (in the absence of a transwell assay). (D) Displacement of NK-92MI cells in the direction of the magnetic gradient (Y axis) after being treated or not with MNPs and exposed to different EMFs. Cell displacement was quantified by analyzing at least 100 cells per video using Imaris software. The results shown (mean ± SD) are representative of three or four independent experiments, *p < 0.05, **p < 0.01, ***p < 0.001.