Table 1. Primers used in this study.

Gene Name	Primers (5’ to 3’)
OsGAMYB-like1	Forward: TCTGAGTTCTTCCTTTCCAATC Reverse: GATACATCGTGCCTCCAGAAA
OsMYB2	Forward: ATGGACATGGCGCACGAGAG Reverse: CACACGGCGGCCTGGGTGG
OsMYBS3	Forward: CCTTTCTGGCAAAATCAGAAAGA Reverse: ATGAACTGGAACAGGCTTGACA
OsMYB4	Forward: CCGTGTGCGCCAAGGA Reverse: GAAGCTGTCGTCGATCTGGAA
OsMYB3R-2	Forward: CAGGGTTTCTATCTCGTTCC Reverse: ATTTCCAAGCCCTTCCAC
OsMYB30	Forward: ACTCCGGGATGGAGATGAG Reverse: GATGAACAGCTTGAGCCAGA
OsMYB55	Forward: ATTACCCTCC ACTCCGTCCT CGGC Reverse: TCCAGTAGTTCTTGATCTCGTTGTC
OsMYB59	Forward: GAGGCGTGCATCAGGAGCCTCG Reverse: GGCGGCGACGAACTGCCGGTG
OsMYB1R	Forward: GAGCAGCATGCAACAGTTTCTC Reverse: GGCTCCCGTTGTAGCTCAAC
OsBMY2	Forward: AGATAAGGGAAAATCGGTTGG Reverse: TTAACAGCGACGGCAAAG
OsBMY6	Forward: TTTCACCCGTAAGACTTA Reverse: AGTGGAAGAAGATTCAGTA
OsBMY10	Forward: TGCCGATTGTCTGAATAAC Reverse: GAGATCCGAGCGATACTT

(Fig. 1a), P. elliottii (Fig. 1b), and O. sativa (Fig. 1c), respectively. Results derived from the experiments of low temperature stress demonstrated that overexpression of OsmiR528 elevates the percentage of cell viability, the rate of cell growth, and reduces the rate of ion leakage in A. thaliana (Fig. 1d, 1g, 1j), P. elliottii (Fig. 1e, 1h, and 1k), and O. sativa (Fig. 1f, 1i, and 1l) at low temperature, respectively.