Skip to main content
. Author manuscript; available in PMC: 2019 Sep 6.
Published in final edited form as: J Vis Exp. 2019 May 3;(147):10.3791/59250. doi: 10.3791/59250

Figure 5: Human NSC derived neurons cultured within chips exhibit excitatory synapses.

Figure 5:

Immunostaining was performed at differentiation day 26. Neuron imaging was performed in the somatic compartment. A) vGlut1 (green) and (B) DAPI (blue) immunolabeling. (C) mCherry-labeled neurons (red) retrograde labeled using a modified rabies virus. (D) A merged fluorescent micrograph of (A-C). (E) Dendritic spines and vGlut1 positive puncta colocalized with mCherry-positive dendrites, shown in a zoomed in region from (D). Immunofluorescence micrographs of (F) neuron specific marker, β-tubulin III (magenta), and (G) vGlut1 (green). (H) Overlay of β-tubulin III, and vGlut1.