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. Author manuscript; available in PMC: 2019 Sep 6.
Published in final edited form as: J Immunol. 2013 Jun 21;191(3):1001–1005. doi: 10.4049/jimmunol.1203520

Figure 1: CD4 T cell reactivity to nonconserved epitopes is selectively diminished following a secondary heterosubtypic influenza infection.

Figure 1:

B10.S and B10 mice were infected sequentially with X-31 (H3N2) followed by x139 influenza (H1N1) or were only infected with x139 influenza. CD4 T cells were isolated from the spleen and EliSpot assays were performed using I-As and I-Ab restricted peptide-epitopes as restimulation antigens. The top panels depict reactivity to conserved epitopes in B10 .S (A) and B10 (B) mice at day 4 (leftward panels) and day 7 or 8 (rightward panels) following infection. Panel C demonstrates CD4 T cell reactivity to nonconserved epitopes in B10.S mice. Each time point represents data from 5–9 individual mice. Panel D shows reactivity to nonconserved epitopes in B10 mice over time; each time point shows the average of 3 individual mice. Results with a p-value of <0.05 using Welch’s T test when comparing the secondary to the primary infection are depicted with an asterisk.