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. 2019 Aug 30;10:917. doi: 10.3389/fphar.2019.00917

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Copyright © 2019 Dondapati, Lübberding, Zemella, Thoring, Wüstenhagen and Kubick

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Repetitive batch expression of NC-hSERT in Sƒ21 system. Protein yields in µg/mL determined by incorporation of [14C] leucine; samples were fractionated, the microsomal fraction (MF) was quantified; different bar colors represent different potassium concentrations in the TT mix; bars show mean protein yield of duplicate measurements with error bars showing the SD. (B) Autoradiography for batch based Expression of NC-hSERT in the Sƒ21 system. Samples were fractionated into translation mix (TM), supernatant 1 (SN) and microsomal fraction (MF).