Table 3.
Significantly altered metabolites (p < 0.05) in the cell line UM-SCC-74B between cells irradiated with 2 Gy [assessed 4 h (rapid) or 24 h (intermediate) after irradiation] vs. non-irradiated cells (control) from the analysis in positive ionization mode are presented as fold changes (95% confidence).
| Metabolite | Rapid vs. control | Intermediate vs. control | Rapid vs. intermediate |
|---|---|---|---|
| POSITIVE IONIZATION | |||
| Phenylalanine | 2.18 ± 1.18 | 3.51 ± 2.18 | |
| Proline | 2.64 ± 1.47 | 3.72 ± 2.12 | |
| Tyrosine | 3.89 ± 2.63 | 8.32 ± 6.08 | |
| Glutamine | 2.62 ± 1.35 | ||
| Methionine | 2.94 ± 1.27 | 3.68 ± 2.23 | |
| Valine | 2.70 ± 1.74 | 5.07 ± 3.53 | |
| Taurine | 2.93 ± 1.38 | 5.20 ± 2.87 | |
| Alanine, beta-alanine, sarcosine | 1.60 ± 0.43 | ||
| Creatine | 4.14 ± 2.81 | 7.64 ± 5.24 | |
| Glucosamine, fructosamine | 4.02 ± 2.89 | ||
| N-Acetyl-glucosamine-1-phosphate | 2.27 ± 1.23 | 3.90 ± 2.50 | |
| Glucosamine-1-phosphate | 2.06 ± 0.75 | 4.90 ± 3.28 | |
| Dihydroneopterin triphosphate | 1.58 ± 0.37 | ||
| Glycerophosphocholine | 5.73 ± 4.13 | 10.56 ± 8.03 | |
| Hydroxyindole | 1.71 ± 0.70 | 2.20 ± 0.96 | |
| Dityrosine | 2.43 ± 1.30 | ||
| Thiamine triphosphate | 2.26 ± 1.19 | 3.81 ± 2.36 | |
| 1-Methylnicotinamide | 2.01 ± 0.88 | ||
| Pantothenic acid | 8.52 ± 7.61 | ||
| UMP | 3.35 ± 1.94 | ||
| NEGATIVE IONIZATION | |||
| Tyrosine | −9.01 ± 2.63 | 7.81 ± 3.92 | |
| Arginine | −4.37 ± 1.92 | ||
| Proline | −17.16 ± 7.94 | ||
| Valine | −23.50 ± 8.91 | ||
| Taurine | −13.71 ± 6.30 | ||
| Alanine, beta-alanine, sarcosine | −28.73 ± 23.88 | ||
| Homoserine, threonine | −39.49 ± 34.97 | ||
| L-Dopa | −3.78 ± 2.15 | ||
| Choline | −5.81 ± 2.33 | 5.06 ± 3.08 | |
| Docosanaoyl-CoA | 45.98 ± 21.19 | ||
| Linolenoyl-CoA | −13.44 ± 11.25 | ||
| Oleoyl-CoA | −7.94 ± 4.77 | ||
| S-Adenosylmethionine | 5.19 ± 3.36 | 4.74 ± 2.96 | |
| Hydantoin-5-propionic acid | −6.50 ± 2.61 | 6.20 ± 3.38 | |
| 1-Arachidonoylglycerophosphoinositol | 3.74 ± 2.01 | ||
| Diphosphoinositol pentakisphosphate | −16.08 ± 3.31 | ||
| Myo-inositol hexakisphosphate | −7.94 ± 2.88 | ||
| Leukotriene E4 | −24.29 ± 14.42 | 5.55 ± 0.38 | |
| Guanine | 6.21 ± 2.41 | −20.50 ± 19.17 | |
| Adenosine | 3.59 ± 2.52 | ||
| Guanosine | 19.83 ± 18.36 | ||
| Pantothenic acid | −19.21 ± 7.12 | ||
| Nicotinic acid mononucleotide | −8.92 ± 3.90 | 7.56 ± 5.20 | |
| 1-methylnicotinamide | −6.75 ± 1.87 | ||
| Niacinamide | −6.34 ± 1.97 | 5.96 ± 2.41 | |
| beta-nicotinamide D-ribonucleotide | −5.11 ± 1.59 | 6.86 ± 4.32 | |
| NAD | −6.50 ± 2.79 | 8.18 ± 5.27 | |
| CMP | 21.64 ± 19.67 | ||
| GMP | 17.49 ± 15.52 | ||
The p-values were calculated using ANOVA followed by a Tukey test and the fold changes were calculated using Fieller's theorem for a 95% confidence interval. A positive fold change indicates a higher level in the radiated cells as compared to the control.