Fig. 2.
Extensive hepatocyte damage by hepatocyte-specific activation of FOXO3 associated with upregulation of HCC-concomitant factors. a More inflammation can be observed in livers from 9-week-old FOXO3CAHep transgenic mice than in control animals. b Expression of Trp53 and Myc by qPCR in livers from 9-week-old control and FOXO3CAHep transgenic mice. c Western blot analysis in livers from 9-week-old control and FOXO3CAHep mice with p53 and c-Myc and GAPDH as loading control (left panel), as well as densitometric quantification (middle and right panels). d Expression of Afp by qPCR in livers from 9-week-old control and FOXO3CAHep transgenic mice (n = 4). e Representative IHC staining for AFP (bar = 100 μm).f Western blot analysis in livers from 9-week-old control and FOXO3CAHep mice with AFP and GAPDH as loading control (upper panel), as well as densitometric quantification (lower panel). g Serum AFP levels in 9-week-old control and FOXO3CAHep mice