Table 2.
Serotypes, stx subtypes and virulence genes of 126 beef cattle STEC isolates
| Serotype | No. of isolates | stx subtypes | Intimin gene | Adherence-related genes | Other virulence-associated genes | Plasmid genes | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| stx 1 | stx 2 | eae | iha | saa | paa | cnf1 | cnf2 | subA | ehxA | espP | katP | ||
| O5(O70):H31 a | 16 | stx 1a | stx 2c | – | + | + | – | – | – | – | + | + | – |
| O5(O70):H31 a | 1 | stx 1a | stx 2d | – | + | + | – | – | – | – | + | + | – |
| O8: H19 | 6 | stx 1a | stx 2a | – | + | + | – | – | – | – | + | + | – |
| O15:H29 | 1 | stx 2d | – | + | – | – | – | – | – | – | – | – | |
| O22:H16 | 2 | stx 2d | – | + | – | – | – | – | – | – | – | – | |
| O65:H19 | 1 | stx 2d | – | + | – | – | – | – | – | – | – | – | |
| O65:H19 | 3 | stx 2c | – | + | – | – | – | – | – | – | – | – | |
| O74:H8 | 1 | stx 1a | stx 2d | – | + | + | – | – | – | – | + | + | – |
| O76:H21 | 1 | stx 2d | – | + | – | – | – | – | – | – | – | – | |
| O81:H29 | 1 | stx 2d | – | + | – | – | – | – | – | – | – | – | |
| O81:H31 | 4 | stx 1a | stx 2a | – | + | + | – | – | – | – | + | + | – |
| O81:H31 | 1 | stx 1a | stx 2a | – | + | + | – | – | – | – | – | – | – |
| O81:H31 | 1 | stx 2a | – | + | + | – | – | – | – | + | + | – | |
| O81:H31 | 3 | stx 1a | stx 2c | – | + | + | – | – | – | – | + | + | – |
| O81:H31 | 1 | stx 1a | stx 2c | – | – | + | – | – | – | – | + | + | – |
| O81:H31 | 14 | stx 1a | stx 2d | – | + | + | – | – | – | – | + | + | – |
| O81:H31 | 1 | stx 1a | stx 2d | + | + | + | – | – | – | – | + | + | – |
| O81:H31 | 1 | stx 1a | stx 2d | – | + | + | + | – | – | – | + | + | – |
| O84:H2 | 28 | stx 1a | + | + | – | + | – | – | – | + | + | – | |
| O84:H2 | 1 | stx 1a | – | + | – | + | – | – | – | + | + | – | |
| O84:H2 | 1 | stx 1a | + | + | – | – | – | – | – | + | + | – | |
| O96:H29 | 3 | stx 2d | – | + | – | – | – | – | – | – | – | – | |
| O116:H10 | 1 | stx 1a | stx 2a | – | + | + | – | – | – | – | + | + | – |
| O145:H12 | 1 | stx 1a | – | + | – | – | + | + | – | – | – | – | |
| O165:H8 | 1 | stx 2a | – | + | + | – | – | – | + | + | + | – | |
| O166:H29 | 1 | stx 2d | – | + | – | – | – | – | – | + | + | – | |
| O166:H29 | 1 | stx 2d | – | + | – | – | – | – | – | – | – | – | |
| O177: HNT | 7 | stx 2c | + | + | – | – | – | – | – | + | + | + | |
| ONT:H8 | 22 | stx 2a | – | + | + | – | – | – | + | + | + | – | |
| Total | 126 | 1 b | 3 b | 37 | 125 | 74 | 30 | 1 | 1 | 23 | 111 | 111 | 7 |
a, These isolates were agglutinated with both O5 and O70 antisera
b, Number of stx1 or stx2 subtypes identified