Fig. 4.

The representative amplification plots and linear standard curves of CCOL2A1 and β-actin genes by RT-qPCR. a The amplification plots and linear standard curve of CCOL2A1 gene; b The amplification plots and linear standard curve of β-actin gene. The upper plots in both a and b show the representative amplification curves including gene positive standards (purple lines) and samples to be tested (other colored lines) with three duplicates per sample in a 96-well plate by RT-qPCR. The X-axis is threshold cycle (Ct), the cycle at which the detected reporter signal was above baseline fluorescence; and Y-axis is ΔRn, the difference of fluorescence between the sample and baseline. The under plots are the representative linear standard curves from pcDNA-CCOL2A1 positive control and β-actin internal control, respectively