Table 1.
The identification of genomic integration of CCOL2A1 gene in vaccinated normal rats on day 42 after vaccination following single intramuscular injection (N = 6)
| Tissues | Mean ± SD (PCN/µg DNA) | |
|---|---|---|
| NS negative control | Vaccinated group | |
| Heart | UD | UD |
| Liver | UD | UD |
| Spleen | UD | UD |
| Lung | UD | UD |
| Kidney | UD | UD |
| Thymus | UD | UD |
| Muscle | UD | 98.65 ± 70.49 |
| Ovary | UD | UD |
| Testis | UD | UD |
| Blood | 157.9 ± 105.1 | 91.63 ± 45.05 |
Values ≤ LOD (182 PCN/µg DNA) indicate a negligible risk of CCOL2A1 gene integration. “UD” respresent “undetermined”. A panel of 10 tissues/organs were harvested and processed for qPCR determination. These data are representative of three experiments. Three separate experiments yielded similar results