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. 2019 Sep 6;17:94. doi: 10.1186/s12951-019-0528-5

Table 2.

The amplified fragment size, primer and probe sequences of CCOL2A1 and β-actin by quantitative polymerase chain reaction

Gene Primer/probe name Prime/probe sequence Fragment size (bp)
Rat β-actin Rat β-actin F-primer 5′-CTCATGCCATCCTGCGTCT-3′ 116
Rat β-actin R-primer 5′-ACGCACGATTTCCCTCTCA-3′
Rat β-actin probe 5′-TGGCCGGGACCTGACAGACTACC-3′
CCOL2A1 CCOL2A1 F-primer 5′-TCTTGTTGGTCCCAGAGGTGA-3′ 118
CCOL2A1 R-primer 5′-ACCCTTGGGTCCGTCAGTG-3′
CCOL2A1 probe 5′-CGTGGATTCCCCGGTGAACGC-3′