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. 2019 Sep 5;16:112. doi: 10.1186/s12985-019-1218-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Construction of the DNA-launched infectious cDNA clone of the DAstV-1 D51 strain. (a) The organization of the viral genome showing the positions of the unique restriction enzyme site used for cloning. ORF1a, ORF1b and ORF2 indicate the DAstV-1 open reading frames. (b, c, d) Fragments A and B were cloned into pEASY-T1 vector, and then cloned into pABX vector producing pABX-AB. (e) Fragment C was fused into pABX-AB releasing pABX-ABC. (f) Lastly, fragment D was cloned into pABX-ABC obtaining the complete genome of DAstV-1 D51