Table 2.
Primers used for construction of D51 infectious clone
| Primer | Sequences(5′-3′) |
|---|---|
| HeadRiboF1 | TTGGCGCGCCACATCATCTGATGAGTCCGTGAGGACGAAACGGTACCCG (Asc I) |
| HeadRiboF2 | CGTGAGGACGAAACGGTACCCGGTACCGTCATCCGAAGTGGGCGAGTCGGGGCCATGGC |
| F1-R | CCAGGTGTCAACAATCATGC |
| Long-7R | CCAGAACATTGTCTTTATTCCCTGTAATCTGTCCAAGTGTGACCACTGTTGTC |
| Long-8F | AGGTGCAGGGTCCCCCTGTCAATGATAAAATGACAACAGTGGTCACACTTGGA |
| aR | CGCGGATCCTTTGGATACGCTGGTGTTG (BamH I) |
| bF | CAGAGAATGCTTGATGAAGGC |
| bR | CGCGGATCCGTTGAAAACTGCCCTGAAGG (BamH I) |
| cF | CTTGGACTGTGGAAGCATATACC |
| cR | CGCGGATCCTCATGTGTGGTGTATACTGTGATG (BamH I) |
| dF | GACAACAGTGGTCACACTTGGA |
| F12-F | ACAGCTGCACTTTCTCATGC |
| HpaR1 | CCGCTCGAGGTCCCATTCGCCATTACCGAGGGGACGGTCCCCTCGGAATGTTGCCCAGCCG (Xho I) |
| HpaR2 | CGGAATGTTGCCCAGCCGGCGCCAGCGAGGAGGCTGGGACCATGCCGGCCTTTT |
| HpaR3 | GGACCATGCCGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATGCCAATTGAA |
| Marker1 | AGCTGGATGTCTGTATTTCGGTTCAACAGCCTCCAGATCTCTCAACAGCGCAC (Bgl II) |
| Marker2 | GCTGTTGTGGTGAAGATCAGTGGTGCGCTGTTGAGAGATCTGGAGGCTGTTGAACC (Bgl II) |
Underlined nucleotides represented the restriction sites