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. 2019 Sep 6;20:351. doi: 10.1186/s12882-019-1534-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Spironolactone (SPL) reduces (a) cytosolic and (b) mitochondrial reactive oxidative species (ROS) production in human aortic endothelial cells (HAECs) stimulated by advanced glycation end products (AGEs) at a concentration of 200 μg/ml. Pre-treatment of AGEs-stimulated HAECs with anti-AGEs neutralizing antibody (Aby) at a concentration of 50 μg/ml or N-acetylcysteine (NAC) at a concentration of 20 mM was used as a positive control. * any groups compared with the vehicle, p < 0.05; $ any groups compared with the AGEs (200 μg/mL)