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. 2019 Sep 6;12:127. doi: 10.1186/s12920-019-0575-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Opsonic antibodies fail to synergize with PTX3 in bolstering the lytic activity of complement towards Bt. Complexes of PTX3 and 10⁷ Bt were subjected to (a) 10% or (b) 25% NHS +/− hyperimmune serum containing anti-Bm antibodies (Ab) also opsonic to Bt. Bacterial CFUs were enumerated at 0, 2, or 6 h post-serum exposure. Two-way ANOVA and Bonferroni multiple comparisons revealed no significant differences in CFUs between PTX3-bound or free bacteria. Data are representative of four to five assays per serum concentration