Fig. 3.

Astilbin attenuated the expression of VEGF. a The protein and mRNA level of VEGF were detected by Western blot and qPCR analysis, *P < 0.05 vs NC group. b Cells were transfected with 10 nM of si-NFE2L2-B or si-NC, the level of Nrf2 was measured by Western blot analysis. c The expression of VEGF were measured by Western blot analysis under treatment with astilbin in Nrf2 knock-down HaCaT cells. d MTT assays showing the effect of Nrf2 knock-down on cell viability under astilbin treatment. **P < 0.005; ns no significance. e The phenotypical presentation of the mice back skin. f Psoriasis-like skin lesions were stained with HE, VEGF antibody. IMQ imiquimod; Low dose, 10 mg/kg; high dose, 50 mg/kg