Table 2.
RT-qPCR to determine Getah virus in mosquito samples in northeastern China in 2018
| Species | No. of mosquitoes | No. of pools (100 mosquitoes/pool) | No. Positive pools | MIR of Mosquitoes (‰)‡ |
|---|---|---|---|---|
| Culex pseudovishnui | 1200 | 12 | 2 | 1.67 (2/1200) |
| Culex tritaeniorhynchus | 7500 | 75 | 12 | 1.60 (12/7500) |
| Anopheles sinensis | 3300 | 33 | 4 | 1.21 (4/3300) |
| Total | 12,000 | 120 | 18 | 1.50 (18/12000) |
†RT-qPCR, quantitative reverse transcription polymerase chain reaction;
‡MIR, minimum infection rate. MIR uses the assumption that a positive pool contains only one infected mosquito the minimum infection rate, which is calculated: ([number of positive pools/total specimens tested] × 1000) (https://www.cdc.gov/westnile/resourcepages/mosqSurvSoft.html)