Fig. 1.

Overview of the photoaffinity pulldown procedure. As indicated, major steps include biotin-containing photo-affinity reagent synthesis and binding to beads, along with tissue or cell lysis, homogenization, and fractionation. Cell lysates are mixed with beads and crosslinked with ultraviolet light, then beads are washed, proteins eluted, and electrophoresis performed. Unique gel bands are identified by a proteomic approach such as peptide mass fingerprinting. Validation experiments subsequent to target identification are not shown.