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. Author manuscript; available in PMC: 2019 Sep 6.
Published in final edited form as: Chem Soc Rev. 2018 May 21;47(10):3433–3469. doi: 10.1039/c7cs00818j

Fig. 19.

Fig. 19

The morphologies of P22 and the mapping of cargo location within them. (A) P22 procapsid (PC) undergoes a morphological change to a more angular expanded (EX) from upon heating, mimicking the structure of the native virion. The internal volume increases approximately 35% and porosity of the capsid decreases. Heating further forms the wiffleball (WB) morphology, where pentamers are removed leaving large holes in the capsid wall. (B) The central sections of three-dimensional reconstructions of the empty P22 and CelB-P22. The darker region on the interior lumen of the capsid indicates higher density, caused by the presence of the bound cargos. The arrows indicate the increased density from the SP-C terminal region of the SP and the CelB-SP binding to the capsid interior. (C) Reconstructions of the EX forms with cargo. Decreased electron density reveals that the cargos are no longer bound to the capsid and can freely move within the entire capsid interior volume. Part A adapted from ref. 261 with permission from The Royal Society of Chemistry. Part B and C reproduced from ref. 260 with permission from The Royal Society of Chemistry.