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. 2004 Aug 11;24(32):7204–7213. doi: 10.1523/JNEUROSCI.2254-04.2004

Figure 1.

Figure 1.

Axonal regrowth from adult map1b+/+ and map1b-/- mice DRG explants in Matrigel. a, b, Immunocytochemical staining for tubulin (Tuj-1 antibody) of map1b+/+ (a) and map1b-/- neurites (b) after 2 d in vitro. Note that the growth pattern of neurites regenerating from map1b-/- DRG appears disoriented and curled (arrows), in contrast to the rather rectilinear growth of map1b+/+ neurites (arrows). Scale bar, 200 μm. c, Mean area occupied by DRG neurites after 1-6 d of growth in vitro (measured as indicated in schematic diagram). Except for the first day, this value is consistently higher in wild-type than in MAP1B knock-out cultures. d, Rectilinear versus curly growth of regenerating neurites. Rectilinearity is represented as the mean ratio between actual neurite lengths and the lengths of straight lines drawn from their emergence point to the tip (as indicated in the schematic diagram). The mostly straight-growing wild-type neurites exhibit an index close to 1, which is significantly higher than that of MAP1B knock-out neurites (***p < 0.001; ANOVA-1), suggesting a correlation between expression of MAP1B and growth orientation.