Table 2.
Association of GluR1 and GluR2 puncta with VGLUT-immunoreactive boutons
|
VGLUT1 |
VGLUT2 |
||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Lamina |
Percentage of boutons with ≥ 1 GluR2 puncta |
Percentage of boutons with ≥ 1 GluR1 puncta |
Mean GluR2 puncta per bouton |
Percentage of GluR2 puncta with GluR1 |
Percentage of boutons with ≥ 1 GluR2 puncta |
Percentage of boutons with ≥ 1 GluR1 puncta |
Mean GluR2 puncta per bouton |
Percentage of GluR2 puncta with GluR1 |
||||||
I | 91.7 | 40.3 | 1.9 | 29.8** | 92 | 16.7 | 1.4 | 12.9 | ||||||
(89-94) | (34-44) | (1.6-2.3) | (23.1-35.0) | (88-100) | (9-24) | (1.1-1.7) | (8.2-17.2) | |||||||
IIo | 95.0 | 64.3 | 2.8 | 40.2 | 97.3 | 24.7 | 1.6 | 17.0* | ||||||
(91-99) | (53-71) | (2.6-3.4) | (30.7-55.0) | (96-100) | (19-28) | (1.4-1.8) | (15.4-19.6) | |||||||
IIi | 98.3 | 76.3 | 4.2 | 38.1** | 96.7 | 24 | 1.6 | 16.9 | ||||||
(97-100) | (73-82) | (3.4-4.7) | (32.7-41.0) | (92-100) | (16-29) | (1.4-1.7) | (11.6-20.6) | |||||||
III | 96.0 | 44.3 | 3.7 | 19.0*** | 95 | 5 | 1.4 | 3.6 | ||||||
(95-97) | (30-60) | (3.0-4.2) | (15.5-24.0) | (90-100) | (4-7) | (1.3-1.5) | (2.7-4.9) | |||||||
IV | 93 | 31.7 | 2.6 | 19.4** | 96.7 | 4.3 | 1.6 | 1.9*** | ||||||
(89-95) | (29-33) | (2.2-3.2) | (14.7-24.9) | (96-98) | (0-11) | (1.4-1.9) | (0-4.3) | |||||||
V | 94 | 21 | 2.6 | 10.4** | 97.7 | 1.7 | 1.5 | 0.8* | ||||||
(91-99) | (16-26) | (2.4-2.9) | (7.3-14.0) | (95-100) | (0-4) | (1.2-1.7) | (0-1.9) | |||||||
IX | 97.3 | 4.3 | 5.5 | 0.8** | 97.7 | 0 | 1.9 | 0 | ||||||
|
(97-98) |
(0-11) |
(3.9-6.4) |
(0-1.8) |
(97-99) |
|
(1.6-2.2) |
|
The proportion of VGLUT1-and VGLUT2-immunoreactive boutons that were adjacent to GluR1 and GluR2 puncta, together with the mean number of GluR2 puncta that contacted each bouton in each group, and the percentage of these that were also GluR1 immunoreactive are shown. One hundred boutons with each type of VGLUT immunoreactivity were examined in each region in three rats. Ranges are given in parentheses. Very few puncta were GluR1 but not GluR2 immunoreactive. The number of these in the sample of 300 VGLUT-immunoreactive boutons in each group is indicated by asterisks as follows: *one; **two; ***three.