Table 3.
Association of GluR1 and GluR2 puncta with CGRP-and IR4-labeled boutons
|
|
CGRP |
IB4 |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lamina |
Percentage of boutons with ≥ 1 GluR2 puncta |
Percentage of boutons with ≥ 1 GluR1 puncta |
Mean GluR2 puncta per bouton |
Percentage of GluR2 puncta with GluR1 |
Percentage of boutons with ≥ 1 GluR2 puncta |
Percentage of boutons with ≥ 1 GluR1 puncta |
Mean GluR2 puncta per bouton |
Percentage of GluR2 puncta with GluR1 |
||||||
| I | 93.3 | 50.3 | 2.6 | 34.4 | ||||||||||
| IIo | (92-94) | (38-58) | (2.4-2.9) | (27.0-42.3) | 100 | 77.3 | 4.8 | 30.7 | ||||||
| IIi |
|
|
|
|
|
(69-93) |
(4.5-5.2) |
(24.9-35.4) |
||||||
The proportion of CGRP-immunoreactive and IB4-labeled boutons that were adjacent to GluR1 and GluR2 puncta, together with the mean number of GluR2 puncta that contacted each bouton in each group, and the percentage of these that were also GluR1 immunoreactive are shown. One hundred boutons of each type were examined from laminae I—IIo (CGRP) or lamina II (IB4) in three rats. Ranges are given in parentheses. In these populations, all GluR1-immunoreactive puncta were also GluR2 positive.