Figure 1.

Protective effects of p53 knock-down in pure neuronal cultures exposed to βAP. a, p53 antisense oligonucleotides (p53-As) increase neuronal survival in cultures exposed to 25 μm βAP(25-35) for 24 hr. Values are means ± SEM of four determinations. ^*p < 0.05 versus controls (CTRL); ^#p < 0.05 versus βAP alone (one-way ANOVA followed by Fisher's LSD test). b, Representative immunoblots of Bax (top) and p53 (bottom) in protein extracts from cortical neurons that have been treated with 25 μm βAP(25-35) for 24 hr in the absence or presence of p53 As or senses (Sn). c, d, Densitometer scan analysis from three determinations. Values represent the means ± SEM of Bax (c) or p53 (d) normalized against β-actin in three independent experiments. ^*p < 0.05 versus controls, or ^#βAP and ^#βAP plus p53-Sn (one-way ANOVA followed by Fisher's LSD test).