Figure 2.

GSK3β inhibitors prevent tau hyperphosphorylation in neurons treated with βAP and z-DEVD. a, Immunoblot of PHF-1 obtained from neuronal cultures treated for 20 hr with 25 μm βAP(25-35) alone or in the presence of 20 μm z-DEVD; 100 μm LiCl or 50 μm TDZD-8 were added 7 hr after βAP. b, Densitometer scan analysis from three determinations. Values represent the means ± SEM of PHF-1 normalized against β-actin in three independent experiments. ^*p < 0.05 versus controls (CTRL), ^#βAP alone, or ^**βAP plusz-DEVD (one-way ANOVA followed by Fisher's LSD test).