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. 2004 Jun 30;24(26):5974–5981. doi: 10.1523/JNEUROSCI.0122-04.2004

Figure 4.

Figure 4.

Absence of conversion of PrP C into PrP Sc after scrapie challenge of MBP-PrP mice. A, Western blots of homogenized brain material electrophoresed natively (-) or after digestion with proteinase K (+). Large amounts of PK-resistant prion protein (PrP Sc) are detectable in brains of wild-type mice (Prnp +/+) that developed terminal scrapie 167 d postinoculation. No PrP Sc is visible in Prnp o/o or MBP-PrP mice that had been challenged intracerebrally with a high dose of the RML strain. Molecular weight markers are indicated on the left. B, Histoblot analysis of PrP Sc expression. Protease-resistant PrP was detectable only in intracerebrally infected Prnp +/+ mice. MBP-PrP and Prnp o/o mice did not accumulate PrP Sc. As reported previously (Brandner et al., 1996b), myelinated structures show faint homogeneous background staining in inoculated Prnp o/o mice.