Figure 4.

Apical type II neurons show features that are intermediate between the rapidly accommodating and slowly accommodating apical type I neurons. A-D show combinations of fluorescent and bright-field images of two neurons from which recordings were made. A, Hoffman optics in combination with the fluorescent image of the Lucifer yellow-filled neuron show the general shape of the neurons on a background of satellite cells. The two neurons from which recordings were made are labeled 1 and 2. B, Hoffman optics in combination with peripherin antibody labeling show that the filled cell is not stained with the type II-specific antibody. C, Fluorescent image showing that peripherin antibody (red) is enriched in the neuron labeled 2 and is essentially absent from the neuron labeled 1. D, Combination of Lucifer yellow and α-peripherin fluorescent images to confirm that the Lucifer yellow dye is located within the peripherin-negative neuron. E, Current-clamp recording from neuron 1. F, Current-clamp recordings from the peripherin-positive neuron labeled 2. G, Current-clamp traces from a neuron outside of the field of view that did not stain with peripherin. The calibration bar, bottom right, applies to E-G.