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. 2004 Apr 28;24(17):4259–4265. doi: 10.1523/JNEUROSCI.5451-03.2004

Figure 2.

Figure 2.

APP43CT-glutathione S-transferase pull-down of LRP-FE65 complex. a, Lysates from LRP-deficient mouse embryonic fibroblast (MEF) cells stably overexpressing LRP-CT were transfected with FE65 (lanes 5, 6) or FE65ΔPID2 expression vector (lanes 3, 4) or untransfected controls (lanes 1, 2). Lysates were incubated with APP43CT-glutathione S-transferase. Bound proteins were coprecipitated by the addition of glutathione-agarose. Proteins were detected with polyclonal anti-LRP Ab 1704. Note that only in the presence of functional FE65 was the LRP-CT fragment pulled down by APP43CT-glutathione S-transferase. The FE65 and FE65ΔPID2 expression in LRP-deficient MEF cells stably overexpressing LRP-CT is shown in b using an Ab against the WW domain in FE65. MW, Molecular weight.