Figure 4.

Time course of the behavioral and endocrine events that occur at larval ecdysis in intact and hormone-injected control (Control) and EH KO(EH-KO)larvae. A, Behaviors expressed at ecdysis by intact larvae (horizontal bars) and levels of ETH-IR and corresponding cGMP-IR in Inka cells from larvae fixed at the times indicated (histograms). Coding of the behavior is that used in Figure 2, except that the average time to tracheal air filling is indicated by a black square above the record of the behavior. B, C, Behavior (horizontal bars) and ETH-IR in Inka cells and corresponding cGMP-IR (histograms), summarized as in A, in larvae injected with ETH1+ETH2 (B, ETH1+ETH2; 1000 fmol each) or 8-Br-cGMP (C, + 8Br-cGMP; 3 pmol). Times indicated are averages ± SEM; n > 8 larvae per data point.