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. 2004 Apr 28;24(17):4266–4282. doi: 10.1523/JNEUROSCI.3688-03.2004

Figure 1.

Figure 1.

RNA transcripts encoding BMP-2 and BMP-4 but not BMP-7 can be detected in both crest-derived (CD) and noncrest-derived (non-CD) cells of the fetal rat gut by RT-PCR. A, BMP expression in the whole bowel and brain (examined as a positive control) at E12 and E14. PCR products were not detected when incubation of extracted RNA with reverse transcriptase (RT) was omitted (-). Note that RNA transcripts encoding BMP-7 were not observed in the bowel at either E12 or E14, although these transcripts were readily detected in the brain. It is thus likely that there is significant expression of BMP-2 and BMP-4 in the E12 and E14 bowel but not BMP-7. B, C, Crest- and noncrest-derived cells in the E14 bowel were separated by positive and negative immunoselection with antibodies to p75NTR. The neural crest marker p75NTR was expressed only in the positively selected crest-derived population, and the mesenchymal marker Hand-1 was expressed only by the negatively selected noncrest-derived population. Immunoselection thus effectively separates crest- and noncrest-derived cells. C, BMP-2 and BMP-4 are expressed both by crest- and noncrest-derived populations of cells.