Figure 3.

Quantitative analysis of Fos expression in VGLUT3-immunoreactive medullary raphe neurons in response to intracerebroventricular injection of saline (open bars; n = 3) or PGE₂ (shaded bars; n = 4) (a) and to exposure of rats to environmental temperature of 24°C (open bars; n = 3) or 4°C (shaded bars; n = 3) (b). The number of immunoreactive cells was counted in every six 20-μm-thick frontal sections of the medulla oblongata (see Fig. 2). Each bar represents the mean ± SEM of the group. Asterisks indicate statistically significant differences between the PGE₂- and saline-injected groups or between the 4°C- and 24°C-exposed groups (p < 0.05).