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. 2004 Jun 9;24(23):5370–5380. doi: 10.1523/JNEUROSCI.1219-04.2004

Figure 4.

Figure 4.

Retrograde transneuronal labeling with PRV inoculated into the interscapular BAT or the tail. a, b, Double immunofluorescence staining for ChAT (green) and PRV (red) in horizontal spinal cord sections of the T3 segment at 47 hr after inoculation into the BAT (a) and of the T12 segment at 98 hr after inoculation into the tail (b). Arrowheads indicate PRV-infected ChAT-immunoreactive neurons clustering in the IML. c, d, Double immunofluorescence staining for VGLUT3 (green) and PRV (red) in rostral medullary raphe regions in frontal sections at 69 hr after inoculation into the BAT (c) and at 121 hr after inoculation into the tail (d). The arrowheads indicate PRV-infected VGLUT3-immunoreactive neurons. GM, Gray matter; WM, white matter. Scale bars: a, b (in b), 100 μm; c, d (in d), 50 μm.