Figure 7.
BAT thermogenesis induced by glutamate microinjection into the IML. a, Changes in temperature of the interscapular BAT (TBAT) after saline or glutamate microinjections into the IML at three sites in segments T3, T4, and T5 (b, circles). TBAT was monitored at intervals of 30 sec. Each value represents the mean ± SEM of three rats per group. The changes in TBAT were significantly different between the glutamate- and saline-injected groups during the time period denoted by a horizontal bar with an asterisk (p < 0.05). b, Composite drawing of the effect of glutamate or saline microinjections into the thoracic spinal cord on TBAT. Each symbol group tied with dotted lines corresponds to sites of microinjections made in one experiment and represents corresponding changes in TBAT 30 min after the microinjections. c, A representative view of a site of intra-IML microinjection. The injection site is clearly identified as a small cluster of fluorescent beads (arrow) in the transverse section counterstained with toluidine blue. DH, Dorsal horn; VH, ventral horn. Scale bar, 500 μm.