Figure 8.
Microinjections of glutamate receptor antagonists into the IML block BAT thermogenesis triggered by bicuculline-induced disinhibition of rostral medullary raphe neurons. a, Changes in TBAT after bicuculline injection into the rRPa in rats microinjected with a mixture of AP-5 and CNQX (n = 4) or saline (n = 3) into the IML over the T2-T6 segments. TBAT was monitored at intervals of 1 min. Each value represents the mean ± SEM of the group. The changes in TBAT were significantly different between the AP-5/CNQX- and saline-pretreated groups during the time period denoted by a horizontal bar with an asterisk (p < 0.05). b, The position of the bicuculline (filled diamonds) and saline (open diamonds) injections made in a. c, A representative view of a site of intra-rRPa injection at the level of interaural -2.30 mm. The injection site is clearly identified as a cluster of fluorescent beads (arrow) in the section counterstained with toluidine blue. 7, Facial nucleus; Giα, alpha part of the gigantocellular reticular nucleus; LPG, lateral paragigantocellular nucleus. Scale bar, 500 μm.