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. 2004 Jun 9;24(23):5283–5291. doi: 10.1523/JNEUROSCI.1224-04.2004

Figure 5.

Figure 5.

Effect of Ant-AIP-II on motor performances, synaptic plasticity, and the NMDA receptor complex of DA-denervated animals. A, A limb-use asymmetry test was performed in sham-operated rats, 6-OHDA denervated animals, and 6-OHDA-lesioned rats receiving a striatal unilateral injection of Ant-AIP-II (n = 8 in each group). 6-OHDA-lesioned rats preferentially use the limb ipsilateral to the lesion (**p < 0.001; 6-OHDA vs sham). Ant-AIP-II peptide reversed the parkinsonian-like motor features in the lesioned animals (not significant, p > 0.05; 6-OHDA plus Ant-AIP-II vs sham). ipsi, Ipsilateral; contra, contralateral. B, Coordinated locomotor activity on a rotarod is significantly impaired after dopamine denervation but recovers to normal levels (i.e., sham group) after unilateral AIP-2 injection (*p < 0.01, 6-OHDA vs sham; not significant, p > 0.05, 6-OHDA plus Ant-AIP-II vs sham). C, Intrastriatal injection of AIP-2 was able to restore normal LTP in 6-OHDA denervated rats (sham, filled circles, p < 0.01, EPSP amplitude after vs before HFS, n = 25; 6-OHDA, open circles, not significant, p > 0.05, EPSP amplitude after vs before HFS, n = 20; 6-OHDA plus Ant-AIP-II, open diamonds, p < 0.01, EPSP amplitude after vs before HFS, n = 20). D, Western blot analysis of active p286-αCaMKII in striatal TIFs from sham- and 6-OHDA-lesioned animals in the absence or presence of 24 hr of Ant-AIP-II treatment (*p < 0.01, 6-OHDA vs sham; **p < 0.01, 6-OHDA plus Ant-AIP-II 24 hr vs 6-OHDA). E, TIF proteins from sham- and 6-OHDA-lesioned animals in the absence or presence of 24 hr of Ant-AIP-II treatment were phosphorylated under conditions known to maximally activate CaMKII and then immunoprecipitated with anti-NR2A-NR2B. Representative autoradiography obtained after immunoprecipitation shows two major phosphorylated protein bands: a 50 kDa band (bottom arrow) pointing to autophosphorylated αCaMKII and a 170 kDa band (top arrow) indicating phosphorylated NR2A-NR2B (*p < 0.005, 6-OHDA vs sham; **p < 0.005, 6-OHDA plus Ant-AIP-II vs 6-OHDA). i.s., Intrastriatal.