Figure 2.

Western blot analysis of total and surface α1 receptor protein. HEK293T cells were transfected with wild-type β2 and γ2 subunits and either wild-type α1 subunit (WT), a 1:1 mixture of wild-type andα1(A322D) subunit (Het),α1(A322D) subunit (Hom), or onlyβ- andγ - subunits but no α1 subunit (βγ). A, Western blots of whole-cell lysates (20 μg of protein) demonstrated a specific immunoreactive band at 50 kDa. B, Transfected HEK293T cells were incubated with sulfo-NHS-SS-biotin, and the biotinylated proteins were analyzed by Western blot.