Figure 4.

Serotonin-induced whole-cell currents from SCG neurons of 5-HT_3A^+/+, 5-HT_3A^vs/+, and 5-HT_3A^vs/vs mice. Whole-cell patch-clamp recordings were obtained from primary cultures of SCG neurons. Representative voltage-clamped current responses from 5-HT_3A^+/+ (left panel), 5-HT_3A^vs/+ (middle panel), and 5-HT_3A^vs/vs (right panel) mice to low (A) and high (B) concentrations of serotonin are shown. The low concentration of serotonin for 5-HT_3A^+/+ and 5-HT_3A^vs/+ SCG neurons was 0.3 μm, and that for 5-HT_3A^vs/vs neurons was 0.1 μm, whereas the high concentration was 10 μm in all three. The number of 5-HT_3A receptors positive over successfully patched neurons for each genotype is shown below each panel.