Figure 7.
Neurogenic-mediated contractions of detrusor smooth muscle from 5-HT3A+/+, 5-HT3Avs/+, and 5-HT3Avs/vs mice of various ages. A, Detrusor strips from male and female 6-week-old 5-HT3A+/+ mice (closed squares and closed circles) responded to electrical field stimulation, whereas bladder strips from male and female 6-week-old 5-HT3Avs/vs mice (open squares and open circles) showed minimal responses to neurogenic-mediated contraction. B, C, At 8 (B) and 12 (C) weeks of age, neurogenic-mediated contractions were completely absent in detrusor strips from male 5-HT3Avs/+ and 5-HT3Avs/vs mice (closed circles and open squares), whereas male 5-HT3A+/+ mice responded to electrical field stimulation (closed squares). Contractile responses were plotted as a percentage of KCl (67 mm)-induced maximal force. Points represent mean contraction ± SEM for n = 3-4 animals per group.