Figure 8.

Effect of carbachol on detrusor smooth muscle contraction in male 5-HT_3A^+/+, 5-HT_3A^vs/+, and 5-HT_3A^vs/vs mice. A, B, Carbachol induced concentration-dependent contractions of bladder smooth muscle in vitro in both 8-week-old (A) and 12-week-old (B) 5-HT_3A^+/+ (closed squares), 5-HT_3A^vs/+ (closed circles), and 5-HT_3A^vs/vs (open squares) mice. C, D, In conscious open filling cystometry, carbachol induced concentration-dependent increases in bladder pressure (BP) in 8-week-old (C) and 12-week-old (D) 5-HT_3A^+/+, 5-HT_3A^vs/+, and 5-HT_3A^vs/vs mice (symbols as above). In both experiments, no differences were seen in carbachol-induced contractions between wild-type and mutant mice, except at the data points indicated (^*). Contraction and bladder pressure changes were plotted as a percentage of KCl (67 mm)-induced maximal force (A, B) and increases from baseline bladder pressure (C, D), respectively. Points represent mean ± SEM for n = 3-6 animals per group. ^*p < 0.05, statistical significance versus 5-HT_3A^+/+ mice.